Table 2.

PCR primers and probes used in this study.

	Primer/probe	Sequence (5′→3')	Product size (bp)	Reference
Circularization of ICE
Tn7100 inverse	Forward	GCGTTAGTGGATCGATCGTAG	508	Hegstad et al., 2020
	Reverse	CACGACGGGTTAAAAACTCA
PCR to confirm transconjugants
aac(6′)-Im	Forward	ACAGATGACCGTGTTCTTGAATTC	143	This study
	Reverse	TGCGTAACCGATAGGAATTGTATC
	Probe	FAM-ACGATTCGTGAGCATTATACAGAGCAATG-BHQ1
mef(E)	Forward	TAATCACTAGTGCCATCCTGCAA	381	This study
	Reverse	ATAGACTGCAAAGACTGACTATAG
	Probe	FAM-ATCGCAGCAGCTGGTGCAGTGCTT-BHQ1
virB4	Forward	GAAGAAGCACAGCAGGATGATA	361	This study
	Reverse	CTTAAATCCTTGTCATCCTGGCA
	Probe	FAM-ATATTAAGCCTATAGGAACTGAGGGAAG-BHQ1
Rd-Rif (Rd-ORF specific PCR)	Forward	TCTAATTATCGGCGCGATTT	463	Hegstad et al., 2020
	Reverse	TCACATCACGATGGAAGGAA