FIG. 1.

Sequence comparison of four cold shock-inducible members of the E. coli CspA family. (A) Amino acid sequence alignments of CspI (database accession no. AE000252), CspG (AE000201), CspB (AE000252), and CspA (AE000433). Residues identical to CspI are shown as dots. The residues forming the hydrophobic core in the β-barrel structure are indicated by solid circles above the sequences. The RNA binding motifs, RNP1 and RNP2, are boxed. Their amino acid sequence homologies are shown on the right, with CspI set at 100%. (B) Sequence alignment of the promoter, 5′-UTR, and the first 13 codon nucleotides of cspI, cspG, cspB, and cspA. Nucleotides identical to cspI are shown as dots. To maximize the alignment, some gaps have been introduced; these are indicated by dashes. The transcription start sites are in bold letters and are marked as +1. The translation start codon ATGs are also in bold letters and are underlined. The most homologous sequences (UP element, -35 region, -10 region, cold box, upstream sequence, Shine-Dalgarno [SD] sequence, and downstream box) are boxed and labeled above the boxes.