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. 2022 Jul 22;13:933249. doi: 10.3389/fmicb.2022.933249

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Copyright © 2022 Thavorasak, Chulanetra, Glab-ampai, Mahasongkram, Sae-lim, Teeranitayatarn, Songserm, Yodsheewan, Nilubol, Chaicumpa and Sookrung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production of recombinant polypeptides of PEDV S1 subunit (S1-0, S1-A, and S1-BCD). (A) PCR amplificons of the DNA sequences coding for the S1-0 (651 bp), S1-A (873 bp), and S1-BCD (660 bp). Lane M, 1 kb DNA ladder. The numbers on the left are DNA sizes in bp. (B) SDS-PAGE-separated patterns of the purified S1-0 (26 kDa), S1-A (32 kDa), and S1-BCD (24 kDa) after staining with CBB dye. (C) Western blot patterns of the purified S1-0, S1-A, and S1-BCD as detected by mouse anti-6× His antibody. Lanes M of (B) and (C) are protein molecular mass markers. The numbers on the left of (B) and (C) are protein molecular masses in kDa.