FIGURE 7.

PLGA MPs trigger autophagic flux in Mtb-infected macrophages. (A) After a total of 24 h, infected macrophages were fixed and stained with anti-LAMP1_and anti-Mtb antibodies, and then observed by laser scanning confocal microscopy. The proportion of (B) LC3 positive Mtb phagosomes and (C) LC3 positive phagosomes which were also positive for LAMP1 were counted. Data represent the mean ± SEM of three independent experiments in which more than 100 phagosomes were counted for each condition. *p < 0.05. The white arrows indicate localization of Mtb phagosomes with GFP-LC3, blue arrows indicate co-localization of Mtb, GFP-LC3, and LAMP1. The results shown are the means of three independent experiments. Reproduced with permission from Lawlor et al. (2016). Copyright © 2016 the authors.