FIG. 2.

Synthesis of AlkB by P. oleovorans during growth on glycerol or glucose. The P. oleovorans cultures, growing exponentially on glycerol (A) or glucose (B), were induced with DCPK at t = 0. At the indicated time points (hours), cell samples were pulse-labeled with [³⁵S]methionine, and AlkB was immunoprecipitated from cell lysates containing 100,000 trichloroacetic acid-precipitable counts. After SDS-polyacrylamide gel electrophoresis analysis and treatment with 1 M sodium salicylate as fluorophore, X-ray films were exposed to the gels at −80°C for 142 h. Molecular mass markers (lane Mw) are indicated in kilodaltons.