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. 1999 Mar;181(5):1610–1616. doi: 10.1128/jb.181.5.1610-1616.1999

FIG. 5.

FIG. 5

Northern blot analysis of total RNA isolated from Pseudomonas and E. coli recombinant cultures with or without 2-h induction of the alk genes with DCPK. Bound DIG-labeled alkB DNA probes were detected with a chemiluminescent substrate. The signal of the blots in panels A, B, and C was recorded on X-ray film by exposure for 5, 2, and 20 min, respectively. The plus and minus signs above the lanes indicate samples taken from induced and uninduced cultures, respectively. RNA molecular size markers of 5.3, 2.8, 1.9, and 1.6 kb (top to bottom, respectively) are indicated in the right margin of each panel. The alkB signal was detected at about 1.5 kb. (A) Lanes 1 and 2, P. putida GPo12 grown on glycerol; lanes 3 and 4, P. oleovorans grown on succinate; lane 5, P. oleovorans grown on glucose; lane 6, P. oleovorans grown on glycerol. (B) Lanes 1 and 2, E. coli W3110(pGEc47) grown on glucose; lanes 3 and 4, E. coli HB101(pGEc47) grown on glucose; lanes 5 and 6, P. oleovorans grown on glucose; lanes 7 and 8, E. coli W3110(pGEc47) grown on pyruvate; lanes 9 and 10, P. oleovorans grown on pyruvate; lane 11, P. oleovorans grown on succinate. (C) Lanes 1 and 2, E. coli W3110(pGEc47) grown on glycerol; lanes 3 and 4, P. oleovorans grown on glycerol; lanes 5 and 6, E. coli HB101(pGEc47) grown on lactate; lanes 7 and 8, P. oleovorans grown on lactate; lanes 9 and 10, E. coli GEc137(pGEc29) grown on glucose.