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. 2022 Aug 5;8(31):eabn4002. doi: 10.1126/sciadv.abn4002

Fig. 5. IFNγ is required for BCG-induced TRIM in vitro.

Fig. 5.

(A) In vitro model to test the effect of IL-18BP and anti-IFNAR1 antibody (Ab) on BCG-induced TRIM. Monocytes were exposed to BCG Denmark (5 μg/ml) for 24 hours (blue line), while the anti-IFNAR1 antibody (10 μg/ml, purple line) and IL-18BP (10 μg/ml, orange line) were in the culture medium for the entire 6 days. At day 6, macrophages were exposed to LPS and cytokine release was measured 24 hours later (day 7). Dot plots show that the cytokine release in picograms per milliliter for (B) TNF and (C) IL-6 at day 7 is elevated in BCG-Mf, with the anti-IFNAR1 antibody having no effect. IL-18BP, on the other hand, completely blocks the LPS response. (D) In vitro model of BCG and inhibitor costimulation for 24 hours only, followed by media for 5 days, before LPS restimulation. (E and F) TNF and IL-6 production is attenuated by 24-hour IL-18BP exposure. *P < 0.05 and **P < 0.01.