Fig. 8. Genes with IFN-associated DMRs show altered expression in human monocytes exposed to BCG in vitro and in mouse HSCs.

(A) Bar plot showing mean DNA methylation change between non–BCG-vaccinated and BCG-vaccinated groups for five DMRs associated with IFN type I response: ADAR, MX1, IFI27, IFITM1, and IRF7. (B) Dot plot showing gene expression of the five genes at day 6 in adult macrophages. All five genes are more highly expressed in BCG-Mf (Denmark-Mf in blue, Bulgaria-Mf in green) compared to Naïve-Mf (gray). (C) Expression of the five genes is altered in mouse HSCs, 4 weeks after intravenous (iv) BCG. Red dots: log₂ fold change in BCG HSCs relative to naïve HSC.