Table 1.

Reverse transcription reaction system details.

Reagent	Volume
5 × HiScript II Buffer	4 μL
dNTP Mix (10 mM each)	1 μL
HiScript II Reverse Transcriptase (200 U/μl)	1 μL
RNase inhibitor (40 U/μl)	1 μL
Oligo (dT) 23VN (50 μM)	1 μL
Random hexamers (50 ng/μl)	1 μL
Total RNA	100 ng
RNase-free distilled deionized water	up to 20 μL