Table 4.
RT-qPCR reaction procedures.
| Item | Reps | Temperature | Time |
|---|---|---|---|
| Predenaturation | 1 | 95 °C | 30 s |
| Cyclic response | 40 | 95 °C | 10 s |
| 60 °C | 30 s | ||
| 95 °C | 15 s | ||
| Dissolution curve | 1 | 60 °C | 60 s |
| 95 °C | 15 s |
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