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Slide culture assay for twitching motility. The P. aeruginosa PAO1 wild type and las and rhl quorum-sensing mutants were inoculated onto slide cultures and incubated for 3 to 5 h at 37°C. The areas in the photomicrographs represent the edges of cell growth (magnification, ca. ×570). (A) PAO1 (wild type); (B) PAO-JP1 (ΔlasI); (C) PDO100 (ΔrhlI); (D) PAO-JP2 (ΔlasI rhlI).
