TABLE 2.
Phage sensitivity, twitching-motility phenotype, and piliation of wild-type and mutant strains
| P. aeruginosa strain | Sensitivity to phage D3112a | Area of twitching motilityb | Surface piliationc |
|---|---|---|---|
| PAO1 | 103 | 20 | 0.407 ± 0.110 |
| PDO100 (rhlI) | 107d | 0e | 0.028 ± 0.009 |
| PAO-JP1 (lasI) | 103 | 12 | 0.351 ± 0.033 |
| PAO-JP2 (lasI rhlI) | 107d | 0 | 0.045 ± 0.014 |
a
Lowest titer of phage stock (dilutions of 103 to 109 PFU/ml were tested) resulting in plaque formation when 10 μl was spotted onto agar overlays containing the strain tested.
b
Diameters (in millimeters) of twitch zones in macroscopic twitch stab assay plates as shown in Fig. 1.
c
Mean A410 values ± standard deviations from ELISAs with PAO1 anti-pilin antibodies against whole cells and alkaline phosphatase-conjugated goat anti-rabbit developing antibodies.
d
Turbid plaques were observed.
e
Very small twitch zones were observed after extended incubation periods (>72 h) in some trials.