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. 2022 Jul 21;11:e78277. doi: 10.7554/eLife.78277

Figure 1. Uterine conditional depletion of Foxa2 causes loss of leukemia inhibitory factor (LIF) secretion and female infertility.

(a) Percentage of mice with pups per total mated mice.Numbers on bars indicate mice with pups over total number of mated mice. *p < 0.05 by Student's t-test. (b) Cre recombinase activity starts differently in Foxa2f/fLtfCre+ and Foxa2f/fPgrCre+ females. (c) Immunostaining of FOXA2 in the uteri on postnatal day 30 and day 4 pregnancy of Foxa2f/f, Foxa2f/fLtfCre+, and Foxa2f/fPgrCre+females. Epithelial cells are outlined by cytokeratin 8 (CK8) staining. Scale bars: 100 μm. (d) In situ hybridization of Lif in day 4 of pregnant uteri from Foxa2f/f, Foxa2f/fLtfCre+, and Foxa2f/fPgrCre+ females. White arrows point to uterine glands. Scale bar: 200 μm. Le, luminal epithelia; Ge, glandular epithelia; S, stroma.

Figure 1.

Figure 1—figure supplement 1. Quantitative PCR of Foxa2 in Foxa2f/f, Foxa2f/fLtfCre+, and Foxa2f/fPgrCre+uteri tissues on day 4 of pregnancy.

Figure 1—figure supplement 1.

RNA levels of Foxa2 are normalized against Rpl7. Values are mean ± SEM; *p < 0.05 by Student's t-test.