Figure 2. Dormant blastocysts are present in Foxa2f/fLtfCre+ and Foxa2f/fPgrCre+uteri on day 8 pregnancy.
(a) Representative photographs of day 8 pregnant uteri from Foxa2f/f, Foxa2f/fLtfCre+, and Foxa2f/fPgrCre+ females. An ovariectomy-induced delayed model of Foxa2f/f mice served as a prototypical control in maintaining dormant blastocysts. Scale bar: 10 mm. Ov, ovary. (b) Blastocysts recovered from Foxa2f/fLtfCre+ and Foxa2f/fPgrCre+uteri on day 8. Blastocysts retrieved from ovariectomized Foxa2f/f mice in delay served as controls. ICM, inner cell mass. Scale bar: 100 µm. Quantification of blastocyst numbers were shown in panel c. Numbers on bars indicate numbers of animals examined. Values are expressed as mean + SEM. (d) Representative photographs of nuclear staining of dormant blastocysts recovered from mice without implantation sites. Scale bar: 50 µm. (e) Average cell numbers per blastocyst. Numbers of embryos examined are shown on bars. Values are expressed as mean + SEM.
Figure 2—figure supplement 1. A schematic outline of sample collection from ovariectomy-induced delayed model.
