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. 2022 Jul 21;11:e78277. doi: 10.7554/eLife.78277

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© 2022, Matsuo, Yuan, Kim et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (a) Schematic outline of sample collection. LIF, LIF administration (20 μg). (b) Representative photograph of uteri from Foxa2^f/fLtf^Cre+ and Foxa2^f/fPgr^Cre+ females (days 6 and 10) with LIF treatment. Foxa2^f/f uteri on day 6 serve as control. Scale bar: 10 mm. Histological pictures of implantation sites in panel b were presented in panel c. Arrowheads point to embryos. Em, embryo. Scale bar: 500 μm. (d) Average number of implantation sites in Foxa2^f/fLtf^Cre+ and Foxa2^f/fPgr^Cre+ mice treated with LIF (20 μg) on day 4 or 8 of pregnancy. Numbers and percentage on bars indicate mice with implantation sites over total number of mated mice. (e) Litter sizes of Foxa2^f/fLtf^Cre+ and Foxa2^f/fPgr^Cre+mice treated with LIF (20 μg) or vehicle on days 4 or 8 of pregnancy. Numbers and percentage on bars indicate mice with pups over total number of mated mice. *p<0.05. (f) 3D visualization of day 6 implantation sites in Foxa2^f/f, Foxa2^f/fLtf^Cre+, and Foxa2^f/fPgr^Cre+females. Images of E-cadherin immunostaining, segmented, and 3D rendered images of day 6 implantation sites in each genotype show defects in Foxa2^f/fPgr^Cre+ females with a LIF injection on day 4 of pregnancy. Scale bar: 200 μm.