Video 4. Nephrin-GFP in larval nephrocyte upon acute Rab5 silencing (surface).

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Confocal live-cell imaging using is shown. Nephrocytes expressing nephrin-GFP (heterozygously) are recorded after 24 hr of acute Rab5 silencing. Increasing gaps and a progressive reduction of slit diaphragms are observed over the observed period. Cells with a mild phenotype were chosen for live-cell imaging to ensure cellular viability. The nephrin signal in tangential sections appears slightly less blurry compared to endogenous nephrin.