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. 2022 Jul 25;11:e77706. doi: 10.7554/eLife.77706

Figure 1. Cytosolic chaperones interact with newly synthesized signal-anchored proteins.

Figure 1.

(A and B) In vitro translation reactions included yeast extracts without mRNA (Ø) or programmed with mRNA encoding HA-tagged variants of signal-anchored proteins (Msp1, Mcr1, Tom20, and Tom70), the tail-anchored protein Fis1, the β-barrel protein Porin, or, as a control, dihydrofolate reductase (DHFR). The reactions were subjected to a pull-down with anti-HA beads. Samples from the input (1%) and the eluates (100%) were analyzed by SDS-PAGE and immunodecoration with the indicated antibodies.

Figure 1—source data 1. Source data for Figure 1A.
Figure 1—source data 2. Source data for Figure 1B.