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. 2022 Jul 25;11:e77706. doi: 10.7554/eLife.77706

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© 2022, Drwesh et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A–C) In-vitro translation reactions included yeast extracts without mRNA (Ø) or programmed with mRNA encoding HA-tagged versions of DHFR or the full length (FL), cytosolic domain (C) or transmembrane segment (T) of the SA proteins: Mcr1 (A), Msp1 (B) and Tom70 (C). The reactions were subjected to a pull-down with anti-HA beads. Samples from the input (1%) and the eluates (100%) were analyzed by SDS-PAGE and immunodecoration with the indicated antibodies.

Figure 2—source data 1. Source data for Figure 2A.

elife-77706-fig2-data1.pdf^{(470.9KB, pdf)}

Figure 2—source data 2. Source data for Figure 2B.

elife-77706-fig2-data2.pdf^{(649.6KB, pdf)}

Figure 2—source data 3. Source data for Figure 2C.

elife-77706-fig2-data3.pdf^{(1.2MB, pdf)}