Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Saccharomyces cerevisiae) | WT | This paper | W303α | |
| Strain, strain background (Saccharomyces cerevisiae) | WT | This paper | JSY7452 | |
| Strain, strain background (Saccharomyces cerevisiae) | tetO7-Ubi-L-ydj1 | This paper | YMK120α, YDJ1::tetO7-Ubiquitin-Leu-YDJ1 KanMX4 | |
| Strain, strain background (Saccharomyces cerevisiae) | tetO7-Ubi-L-sis1 | This paper | YMK120α, SIS1::tetO7-Ubiquitin-Leu-SIS1 His3MX |
|
| Strain, strain background (Saccharomyces cerevisiae) | tetO7-Ubi-L-ydj1/sis1 | This paper | YMK120α, tetO7-Ubi-Leu-SIS1:HisMX3a; tetO7-Ubi-Leu-YDJ1:KanMX4 | |
| Strain, strain background (Saccharomyces cerevisiae) | tom20Δ | This paper | W303α, TOM20::HIS3 | |
| Strain, strain background (Saccharomyces cerevisiae) | tom70/71Δ | This paper | JSY7452, TOM70::TRP1, TOM71::HIS3 | |
| Sequence-based reagent | Msp1 Fwd | This paper | PCR primers | GGGGGATCCATGTCTCGCAAA TTTGATTTAAAAACGATTACT GATCTTT |
| Sequence-based reagent | Msp1 Rev | This paper | PCR primers | GGGAAGCTTATCAAGAGGTTGA GATGACAACGTACTTG |
| Sequence-based reagent | yk Msp1 Fwd | This paper | PCR primers | GGGGGATCCAAAAAAATGT CTCGCAAATTTGATTTAAAA ACGATTACTGATCTTT |
| Sequence-based reagent | Yk Msp1 Rev | This paper | PCR primers | GGGAAGCTTTTAATCAAGA GGTTGAGATGACAAC |
| Sequence-based reagent | yk Msp1-3HA Fwd | This paper | PCR primers | CACACGAGCTCAAAAAAA TGTCTCGCAAATTTGATTTAA AAACG |
| Sequence-based reagent | yk Msp1-3HA Rev | This paper | PCR primers | CACACGGATCCCCATCAAG AGGTTGAGATGACAACGTAC |
| Sequence-based reagent | yk Mcr1-3HA Fwd | This paper | PCR primers | GGGGAATTCAAAAAAATGT TTTCCAGATTATCCAGATCTCACTCAAAAGC |
| Sequence-based reagent | yk Mcr1-3HA Rev | This paper | PCR primers | GGGCCCGGGAAATTTG AAAACTTGGTCCTTGGAGTAG CCC |
| Sequence-based reagent | yk Tom20-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAATG TCCCAGTCGAACCCTATCT TAC |
| Sequence-based reagent | yk Tom20-3HA Rev | This paper | PCR primers | GGGGGATCCGGGTCA TCGATATCGTTAGCTTCAGC |
| Sequence-based reagent | yk Tom70-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAAT GAAGAGCTTCATTACAAGGA ACAAGAC |
| Sequence-based reagent | yk Tom70-3HA Rev | This paper | PCR primers | GGGGGATCCGGCATTAA ACCCTGTTCGCGTAATTTAGC |
| Sequence-based reagent | yk Msp1-TMD-3HA Fwd | This paper | PCR primers | GGGGAATTCAAAAAAA TGTCTCGCAAATTTGATTTAA AAACG |
| Sequence-based reagent | yk Msp1-TMD-3HA Rev | This paper | PCR primers | GGGGGATCCCCGTT GAGTAGCCGACTGACCA |
| Sequence-based reagent | yk Msp1-CD-–3HA Fwd | This paper | PCR primers | CACACGAGCTCAAAAAAA TGGATGTTGAATCAGGACCGTTATCAGG |
| Sequence-based reagent | yk Msp1-CD-–3HA Rev | This paper | PCR primers | CACACGGATCCCCATCAAG AGGTTGAGATGACAACGTAC TTGTAGC |
| Sequence-based reagent | yk Mcr1-TMD-3HA Fwd | This paper | PCR primers | CACACGAATTCAAAAAAA TGTTTTCCAGATTATCCAG ATCTC |
| Sequence-based reagent | yk Mcr1-TMD-3HA Rev | This paper | PCR primers | CACACCCCGGGGACAAAGG AATGTTGGTTACG GTTT |
| Sequence-based reagent | yk Mcr1-CD-3HA Fwd | This paper | PCR primers | CACACGAATTCAAAAAAAT GCATTCCTTTGTCTTCAATG AATC |
| Sequence-based reagent | yk Mcr1-CD-3HA Rev | This paper | PCR primers | CACACCCCGGGAAATTTGA AAACTTGGTCCTTGGAGTAG |
| Sequence-based reagent | yk Tom20-TMD-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAATGT CCCAGTCGAACCCTATCTTAC |
| Sequence-based reagent | yk Tom20-TMD-3HA Rev | This paper | PCR primers | GGGGGATCCGGGTCAAA GTAGATAGCATAACCGGTG |
| Sequence-based reagent | yk Tom20-CD-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAAT GAGAAATAGCCCGCAATTC AGGAA |
| Sequence-based reagent | yk Tom20-CD-3HA Rev | This paper | PCR primers | GGGGGATCCGGGTCATC GATATCGTTAGCTTCAGC |
| Sequence-based reagent | yk Tom70-TMD-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAA TGAAGAGCTTCATTACAAGGA ACAAGAC |
| Sequence-based reagent | yk Tom70-TMD-3HA Rev | This paper | PCR primers | GGGGGATCCGGCAATTGGT TGTAATAATAGTAGGCACC |
| Sequence-based reagent | yk Tom70-CD-3HA Fwd | This paper | PCR primers | GGGGGTACCAAAAAAATGC AACAACAACAACGAGGAAAAAAGAACAC |
| Sequence-based reagent | yk Tom70-CD-3HA Rev | This paper | PCR primers | GGGGGATCCGGCATTAAACC CTGTTCGCGTAATTTAGC |
| Sequence-based reagent | tetO7-Ubi-L-Ydj1 Fwd | Jores et al., 2018 | PCR primers | CATATCTTTTGATAGAACATA ATTAAAAATTATCCAAACTGA ATTCTACACAGTATAGCGACC AGCATTCACATACG |
| Sequence-based reagent | tetO7-Ubi-L-Ydj1 Rev | Jores et al., 2018 | PCR primers | GTGGCAGTTACTGGAACACC TAGAATATCGTAAAACTTAG TTTCTTTAACCAAACCACCTC TCAATCTCAAGACCAAG |
| Sequence-based reagent | tetO7-Ubi-L-Sis1 Fwd | Jores et al., 2018 | PCR primers | GGATAAGTTGTTTGCATTTTA AGATTTTTTTTTTAATACATT CACATCAACAGTATAGCGAC CAGCATTCACATACG |
| Sequence-based reagent | tetO7-Ubi-L-Sis1 Rev | Jores et al., 2018 | PCR primers | TTAGCACTTGGAGATACT CCAAGTAAATCATAAAGTTT TGTCTCCTTGACCAAACCACC TCTCAATCTCAAGACCAAG |
| Recombinant DNA reagent | pGEM4polyA-3HA (plasmid) | Jores et al., 2018 | C-terminal 3 x HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-DHFR-3HA (plasmid) | Jores et al., 2018 | Yeast kozak sequence (AAAAAAATG) DHFR-3 ×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Porin-3HA (plasmid) | Jores et al., 2018 | Yeast kozak sequence (AAAAAAATG) Porin-3 ×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Fis1-3HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Fis1−3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Msp1-3HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Msp1−3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Mcr1-3HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Mcr1−3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom20-3HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom20−3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom70-3HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom70−3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Msp1(33-363)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Msp1(1-363)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Msp1(1-32)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Msp1(1-32)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Mcr1(35-302)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Mcr1(35-302)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Mcr1(1-39)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Mcr1(1-39)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom20(33-183)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom20(33-183)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom20(1-30)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom20(1-30)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom70(33-617)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom70(33-617)–3×HA-tag | |
| Recombinant DNA reagent | pGEM4polyA-yk-Tom70(1-32)–3 HA (plasmid) | This paper | Yeast kozak sequence (AAAAAAATG) Tom70(1-32)–3×HA-tag | |
| Recombinant DNA reagent | pMK632His (plasmid) | Jores et al., 2018 | HIS3MX cassette tetO7-CYC1 promoter-Ubiquitin-Leucin-HA-tag | |
| Recombinant DNA reagent | pMK632Kan (plasmid) | Jores et al., 2018 | KanMX cassette tetO7-CYC1 promoter-Ubiquitin-Leucin-HA-tag | |
| Recombinant DNA reagent | pGEX4T1-GST (plasmid) | This paper | GST | |
| Recombinant DNA reagent | pGEX4T1-GST-Tom20(35-183) (plasmid) | This paper | Tom20(35-183) | |
| Recombinant DNA reagent | pGEX4T1-GST-Tom70 (46-617) (plasmid) | This paper | Tom70(46-617) | |
| Recombinant DNA reagent | pPROEX-HTa-cBag (plasmid) | Young et al., 2003 | His6-tag-TEV-human Bag-1M(151-263) | |
| Recombinant DNA reagent | pPROEX-HTa-(C90) (plasmid) | Young et al., 2003 | His6-tag-TEV-human Hsp90a(566-732) | |
| Antibody | Anti-Ssa1/2 (rabbit polyclonal) | Jores et al., 2018 | 1:20,000 | |
| Antibody | Anti-Ydj1 (rabbit polyclonal) | Jores et al., 2018 | 1:10,000 | |
| Antibody | Anti-Sis1 (rabbit polyclonal) | Jores et al., 2018 | 1:20,000 | |
| Antibody | Anti-Hsp26 (rabbit polyclonal) | Jores et al., 2018 | 1:4000 | |
| Antibody | Anti-Hsp104 (rabbit polyclonal) | Jores et al., 2018 | 1:25,000 | |
| Antibody | Anti-Hsp42 (rabbit polyclonal) | Jores et al., 2018 | 1:4000 | |
| Antibody | Anti-Hsp82 (rabbit polyclonal) | Jores et al., 2018 | 1:20,000 | |
| Antibody | Anti-Hch1 (rabbit polyclonal) | Jores et al., 2018 | 1:4000 | |
| Antibody | Anti-Bmh1 (rabbit polyclonal) | This paper | 1:1000 | |
| Antibody | Anti-Djp1 (rabbit polyclonal) | Lab of Ineke Braakman | 1:2000 | |
| Antibody | Anti-Sti1 (rabbit polyclonal) | This paper | 1:10,000 | |
| Antibody | Anti-Aha1 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Msp1 (rabbit polyclonal) | Lab of Toshiya Endo | 1:2000 | |
| Antibody | Anti-Mcr1 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Fis1 (rabbit polyclonal) | This paper | 1:1000 | |
| Antibody | Anti-Tom20 (rabbit polyclonal) | This paper | 1:4000 | |
| Antibody | Anti-Tom70 (rabbit polyclonal) | This paper | 1:5000 | |
| Antibody | Anti-Porin (rabbit polyclonal) | This paper | 1:6000 | |
| Antibody | Anti-Pic2 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-HA (rat polyclonal) | Roche | #11867423001 | 1:1000 |
| Antibody | Anti-Cor1 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Mim1 (rabbit polyclonal) | This paper | 1:100 | |
| Antibody | Anti-Cox2 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Oxa1 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Erv1 (rabbit polyclonal) | Lab of Johannes Herrmann | 1:1000 | |
| Antibody | Anti-Aco1 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Tom22 (rabbit polyclonal) | This paper | 1:2000 | |
| Antibody | Anti-Om14 (rabbit polyclonal) | Lab of Thomas Becker | 1:2000 | |
| Antibody | Goat Anti-Rabbit IgG HRP conjugate | Bio-Rad | #1721019 | 1:10,000 |
| Antibody | Goat Anti-Rat IgG HRP conjugate | Abcam | #ab6845 | 1:2000 |