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. 2022 Aug 5;11:e75863. doi: 10.7554/eLife.75863

Figure 4. In vivo knockdown of Septin7 alters myofibrillar structure.

(A, B) Representative electron microscopy (EM) images of myofibrils from cross-sectional samples of m. tibialis anterior (TA) muscles from Cre- and Cre+ mice at smaller (left) and larger (right) magnification where scale bars represent 1 μm. (C, D) Area and perimeter of the individual myofibrils and the number of myofibrils within 1 µm2 of an appropriate visual field were determined from EM images. Here and in all subsequent figures, green columns represent data from Cre-, while red columns from Cre+ animals (average ± SEM). The total number of myofibrils counted for area and perimeter was 3012 and 3174 in Cre- and Cre+ mice, respectively, while the number of visual fields examined was 72 and 74, for calculating the number of myofibrils (*p<0.05; ***p<0.001 from t-test). See also Figure 4—figure supplement 1.

Figure 4.

Figure 4—figure supplement 1. Changes in myofibrillar parameters with in vivo knockdown of Septin7.

Figure 4—figure supplement 1.

Myofibrillar parameters calculated from cross-sectional electron microscopy (EM) pictures of m. tibialis anterior (TA) muscles of control BL6 (black) and Cre- (green) mice. Histograms represent the distribution of mitochondrial area (A) and perimeter (B) in control and Cre- samples, respectively. (C) Average of mitochondrial area and perimeter (average ± SEM). The number of individual myofibrils (for area and perimeter) was 1916 and 3012, respectively (D). The number of myofibrils within 1 µm2 of visual field in control BL6 and Cre- mice. The number of visual fields used for the calculation of myofibrillar data was 42 and 72 in samples originated from BL6 and Cre- mice, respectively.