Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | Human reference genome NCBI | Genome Reference Consortium | http://www.ncbi.nlm.nih.gov/projects/genome/assembly/human | |
| Gene (Mus musculus) | Mouse reference genome NCBI | Genome Reference Consortium | http://www.ncbi.nlm.nih.gov/projects/genome/assembly/mouse | |
| Strain, strain background (Escherichia coli) | JM 109 competent E. coli cells | Promega | Cat# L2005 | |
| Strain, strain background (M. musculus) | B6.Cg-Tg(ACTA1-cre)79Jme/J mice (ssC0) | Jackson Laboratory | Cat#006149; RRID:IMSR_JAX:006149 | |
| Strain, strain background (M. musculus) | C57BL6 Septin7flox/flox (SS00) mice | Prof. Dr. Matthias Gaestel | Institute of Physiological Chemistry Hannover Medical School | |
| Cell line (M. musculus) | Mouse immortalized C2C12 | ATCC | Cat# CRL-1772; RRID:CVCL_0188 | |
| Biological sample (Homo sapiens) | Human m. quadriceps femoris | Kenézy Gyula Teaching Hospital of the University of Debrecen | https://kenezykorhaz.unideb.hu/en | |
| Antibody | Anti-Septin7 (rabbit monoclonal) | IBL | Cat# JP18991; RRID:AB_1630825 | IF (1:200), WB (1:250) |
| Antibody | Anti-α-actinin (mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-17829; RRID:AB_626633 | WB (1:250) |
| Antibody | Anti-Pax7 (mouse monoclonal) | Sigma | Cat# SAB1404168; RRID:AB_10738723 | IF (1:200), WB (1:200) |
| Antibody | Anti-desmin (rabbit polyclonal) | Sigma | Cat# D8281; RRID:AB_476910 | IF (1:200), WB (1:250) |
| Antibody | Anti-Ryanodin receptor 1 (mouse monoclonal) | Thermo Fisher Scientific | Cat# MA3-925; RRID:AB_2254138 | IF (1:250) |
| Antibody | Anti-α-actinin (skeletal muscle specific) (mouse monoclonal) | Sigma | Cat# A7811; RRID:AB_476766 | IF (1:500) |
| Antibody | Anti-myosin4 (MYH4; MF20) (mouse monoclonal) | Thermo Fisher Scientific | Cat# 14-6503-80 | IF (1 µg/ml) |
| Antibody | Anti-L-type calcium channel (rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-16229-R | IF (1:250) |
| Antibody | HRP-conjugated anti-rabbit (goat monoclonal) | Bio-Rad Laboratories | Cat# 170-6515; RRID:AB_11125142 | WB (1:500) |
| Antibody | HRP-conjugated anti-mouse (goat monoclonal) | Bio-Rad Laboratories | Cat# 170-6516; RRID:AB_11125547 | WB (1:500) |
| Antibody | Alexa Fluor 488 conjugated anti-rabbit IgG (goat monoclonal) | Thermo Fisher Scientific | Cat# A32731; RRID:AB_2633280 | IF (1:500) |
| Antibody | Cyanine3 conjugated anti-Mouse IgG (goat monoclonal) | Thermo Fisher Scientific | Cat# A10521; RRID:AB_1500665 | IF (1:500) |
| Recombinant DNA reagent | Septin7-specific CRISPR/Cas9 KO plasmid constructs | Santa Cruz Biotechnology | Cat# sc-433427 | |
| Recombinant DNA reagent | Septin7-specific HDR plasmid constructs | Santa Cruz Biotechnology | Cat# sc-433427-HDR | |
| Recombinant DNA reagent | Septin7-specific shRNA constructs in retroviral pGFP-V-RS vectors | Origene | Cat# TR30007 | |
| Commercial assay or kit | Omniscript RT kit | QIAGEN | Cat# 205113 | |
| Commercial assay or kit | RNase free DNase kit | Thermo Fisher Scientific | Cat# EN0521 | |
| Commercial assay or kit | SYBRGreen mix | PCR Biosystems | Cat# PB20.11-51 | |
| Commercial assay or kit | High-specificity TaqMan assays | Thermo Fisher Scientific | Cat# 4331182 | |
| Commercial assay or kit | CyQUANT NF Cell Proliferation Assay Kit | Invitrogen | Cat# C35006 | |
| Commercial assay or kit | SuperSignal West Pico PLUS Chemiluminescent Substrate | Thermo Fisher Scientific | Cat# 34577 | |
| Chemical compound, drug | Tamoxifen containing chow | Envigo | Cat# TD 130857 | |
| Chemical compound, drug | Type I collagenase | Sigma | Cat# SCR103 | |
| Chemical compound, drug | Isoflurane | Forane | Cat# NDC 10019-360 | |
| Chemical compound, drug | Durcupan epoxy resin | Sigma | Cat# 44611 | |
| Chemical compound, drug | EZ-Vision Dye 6X | VWR Life Science | Cat# 97064-190 | |
| Chemical compound, drug | MitoTracker Red CMXRos | Thermo Fisher Scientific | Cat# M7512 | (0.5 µM) |
| Chemical compound, drug | Lipofectamine 2000 | Thermo Fisher Scientific | Cat# 11668019 | |
| Chemical compound, drug | Opti-MEM Reduced Serum medium | Thermo Fisher Scientific | Cat# 31985070 | |
| Software, algorithm | Nucline and InterView FUSION software | Mediso Ltd. | http://ctamed.com/en/portfolio/software-solutions/#fusion | |
| Software, algorithm | Axotape software | Axon Instruments | https://www.bioz.com/result/axotape/product/Molecular%20Devices%20Llc | |
| Software, algorithm | Primer Premier 5.0 software | Premier Biosoft | http://downloads.fyxm.net/Primer-Premier-101178.html | |
| Software, algorithm | Program to design target-specific primers for PCR | Primer-BLAST | https://www.ncbi.nlm.nih.gov/tools/primer-blast/ | |
| Software, algorithm | Statistical program Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | Image processing program ImageJ |
Image J1.40g | https://imagej.nih.gov/ij/download.html | |
| Software, algorithm | Origin 8.6 | OriginPro | https://originpro.informer.com/8.6/ | |
| Other | DAB substrate kit | Thermo Fisher Scientific | Cat# 34002 | Histological labeling reagent used for the detection of Pax7 expression on muscle sections following BaCl2-induced injury |
| Other | Protein blocking solution serum-free | Dako | Cat# X0909 | Reagent used for blocking unspecific binding sites of the samples before application of primary antibodies in fluorescent immunolabeling procedure |
| Other | TRITC-phalloidin | Sigma | Cat# P1951 | Fluorescently labeled actin-specific labeling IF (1:1000) |
| Other | FITC-phalloidin | Sigma | Cat# P5282 | Fluorescently labeled actin-specific labeling IF (1:1000) |
| Other | Mowiol 4-88 | Sigma | Cat# 81381 | Mounting medium used to cover fluorescently labeled samples until the microscopic analysis |
| Other | FACSAria flow cytometer | BD Biosciences | https://www.bdbiosciences.com/en-eu/instruments/research-instruments/research-cell-sorters/facsaria-iii | Device used for single cell sorting based on the expression of fluorescent proteins (GFP and RFP) |
| Other | nanoScan SPECT/CT | Medisol Ltd | http://scanomedtranslational.com/ | Device used for whole body CT scans of mice |
| Other | Mouse running wheel | Campden Instruments Ltd Bodnár et al., 2014 |
Device used to measure the voluntary activity of mice | |
| Other | Capacitive mechano-electric force transducer | Experimetria Bodnár et al., 2016 |
Device used to measure in vitro muscle force | |
| Other | Leica Ultracut UCT | Leica Microsystems | https://www.leica-microsystems.com | Device used to cut ultrathin sections from muscle samples for EM analysis |
| Other | JEM1010 transmission electron microscope | JEOL | https://www.jeolusa.com/PRODUCTS/Transmission-Electron-Microscopes-TEM | Device used to examine ultrathin muscle sections at high magnification |
| Other | HT Mini homogenizer | OPS Diagnostics | Cat# BM-D1030E | Device used to homogenize muscle samples for protein analysis. |
| Other | Labnet MultiGene 96-well Gradient Thermal Cycler | Labnet International | Cat# TC9610-230 | Device used for RT-PCR reactions |
| Other | LightCycler 480 | Roche | Cat# 05015243001 | Device used for quantitative PCR measurements |
| Other | FlexStation 3 multimode microplate reader | Molecular Devices | Cat# Flex3 | Device used to measure fluorescent signal of CyQUANT cell proliferation assay |
| Other | AiryScan Confocal microscope | Zeiss | https://www.zeiss.com/microscopy/int/dynamic-content/news/2014/news-lsm-880.html | Device used to examine fluorescent immunolabeling of muscle fibers and cultured cells at high spatial resolution |