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. 2022 Jun 23;28(14):3076–3090. doi: 10.1158/1078-0432.CCR-22-0486

Figure 6.

Figure 6. Anti-VEGFR2 antibody treatment at 5 mg/kg increases intravascular and interstitial pO2 while restoring perfusion (A–D), whereas losartan increases interstitial pO2 on day 5 after reperfusing vessels on day 2 in E0771 (E–J). A, Measurements of intravascular oxygen tension in mmHg in control IgG and anti-VEGFR2 (DC101) (both 5 mg/kg i.p. every 3 days) antibody-treated mice at baseline, day 2, and day 5. n = 64–103 measurements per time point per group. N = 5–8 mice per group in the DC101 studies. Lines indicate statistically significant differences between multiple comparisons after a Kruskal–Wallis test with Dunn's correction, with differences depicted only for comparisons between control and treatment groups on the same day. B, Extravascular (interstitial, i.e., measurements 40–60 μm from the nearest vessel) oxygen measurements in the same tumors. n = 246–367 measurements per time point per group. C, Measurements of extravascular oxygen tension in mmHg normalized to the intravascular oxygen tension of blood vessels. D, Distance to the nearest vessel measurements in the same tumors. n = 870–1,962 measurements per time point per group. E, Measurements of intravascular oxygen tension in mmHg in control PBS and losartan-treated mice at baseline, day 2, and day 5. n = 12–38 measurements per time point per group. N = 4–6 mice per group. F, Extravascular (interstitial, i.e., measurements 40–60 μm from the nearest vessel) oxygen measurements in the same tumors. n = 42–161 measurements per time point per group. N = 4–6 mice per group. G, Distance to the nearest vessel measurements in the same tumors. n = 412–1,279 measurements per time point per group. H, Scatter plot of interstitial oxygen tension versus the diameter of the nearest vessel in E0771 control-treated tumors on day 5. Correlation coefficient and P value are denoted on the graph. I, Scatter plot of interstitial oxygen tension versus the diameter of the nearest vessel in E0771 losartan-treated tumors on day 5. Correlation coefficient and P value are denoted on the graph. J, Measurements of extravascular oxygen tension in mmHg normalized to the intravascular oxygen tension of blood vessels.

Anti-VEGFR2 antibody treatment at 5 mg/kg increases intravascular and interstitial pO2 while restoring perfusion (A–D), whereas losartan increases interstitial pO2 on day 5 after reperfusing vessels on day 2 in E0771 (E–J). A, Measurements of intravascular oxygen tension in mm Hg in control IgG and anti-VEGFR2 (DC101) (both 5 mg/kg i.p. every 3 days) antibody-treated mice at baseline, day 2, and day 5. n = 64–103 measurements per time point per group. N = 5–8 mice per group in the DC101 studies. Lines indicate statistically significant differences between multiple comparisons after a Kruskal–Wallis test with Dunn's correction, with differences depicted only for comparisons between control and treatment groups on the same day. B, Extravascular (interstitial, i.e., measurements 40–60 μm from the nearest vessel) oxygen measurements in the same tumors. n = 246–367 measurements per time point per group. C, Measurements of extravascular oxygen tension in mm Hg normalized to the intravascular oxygen tension of blood vessels. D, Distance to the nearest vessel measurements in the same tumors. n = 870–1,962 measurements per time point per group. E, Measurements of intravascular oxygen tension in mm Hg in control PBS and losartan-treated mice at baseline, day 2, and day 5. n = 12–38 measurements per time point per group. N = 4–6 mice per group. F, Extravascular (interstitial, i.e., measurements 40–60 μm from the nearest vessel) oxygen measurements in the same tumors. n = 42–161 measurements per time point per group. N = 4–6 mice per group. G, Distance to the nearest vessel measurements in the same tumors. n = 412–1,279 measurements per time point per group. H, Scatter plot of interstitial oxygen tension versus the diameter of the nearest vessel in E0771 control-treated tumors on day 5. Correlation coefficient and P value are denoted on the graph. I, Scatter plot of interstitial oxygen tension versus the diameter of the nearest vessel in E0771 losartan-treated tumors on day 5. Correlation coefficient and P value are denoted on the graph. J, Measurements of extravascular oxygen tension in mm Hg normalized to the intravascular oxygen tension of blood vessels.