Figure 4.

Functional classification of differentially expressed unigenes (DEGs). (A) Hierarchical clustering heatmap of DEGs. Each column and row represent a sample and a DEG, respectively, and the colors indicate the expression levels of DEGs. SCC and WCC, summer and winter Chinese cedar needle samples, respectively, and SCC_1, SCC_2, and SCC_3 represent 3 repetitions. (B) Volcano plot of DEGs. Each point in the volcano plot represents a unigene, the abscissa represents the logarithm of the quantitative difference multiples of a unigene in two samples, and the ordinate represents the significance level of DEGs. The blue/red dots in the figure represent down/upregulated DSMs, and the gray dots represent metabolites that were detected but not significant. (C) Comparison of the distribution of DEGs at the GO level. Red/green indicates GO entries enriched for up/downregulated DEGs; the x-axis is the name of the entry, and the y-axis represents the number of unigenes in the corresponding entry and their percentages. KEGG enrichment analysis of downregulated (D) and upregulated (E) DEGs in WCC needles. The x- and y-axes represent the enrichment score and pathway term, respectively. The colors and sizes of the dots represent the significance (p) and number of unigenes, respectively.