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. 2022 Jul 22;13:937466. doi: 10.3389/fmicb.2022.937466

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Copyright © 2022 Garcia-Moreno, Jordan, Günther, Dau, Fritzsch, Vermes, Schoppa, Ignatius, Wildemann, Werz, Löffler and Tuchscherr.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR2 expression is not altered in infected osteocytes. Influence of Staphylococcus aureus on osteoblasts and osteocytes surface TLR2 expression. Expression of TLR2 among living cells was analyzed by flow cytometry. The mean fluorescence intensity (MFI) of TLR2 was determined. Statistics are calculated with raw data (MFI), infected vs. uninfected and a statistical analysis was performed using the unpaired t-test for each pair unpaired t-test; *p < 0.05. The bars and whiskers represent the means ±SD of different independent experiments; n = 3. −: control cells and +: infected cells.