Figure 5.

Proteomic analysis of osteoblasts and osteocytes infected with the Staphylococcus aureus LS1 strain. At 24 h post infection, the supernatants of infected and non-infected cells were collected and analyzed by mass spectrometry. (A) Principal component analysis (PCA) score plot comparing metabolic profiles of infected and non-infected osteoblasts and osteocytes. (B–D) Volcano plot (q-value vs. log₂ fold change) for differentially expressed proteins: (B) Infected vs. non-infected osteoblasts. (C) Infected vs. non-infected osteocytes. (D) Infected osteoblasts vs. infected osteocytes. The X axis represents −log10Q value (values of p < 0.05); the Y axis represents log₂ values of protein fold changes. See details in Supplementary Tables S3–5; n = 3.