Figure 6.

Antimicrobial response and cytotoxicity induced by Staphylococcus aureus in osteoblasts and osteocytes. (A) Secretion of LL-37 was measured by ELISA in osteoblasts and osteocytes cell free supernatants, 24 h after infection (ng/10⁶cells). Uninfected values were subtracted from infected values; n = 3. (B) % Cell death induced by S. aureus in osteoblasts and osteocytes at different doses (MOIs). Twenty-four hours post infection, cell death was measured by flow cytometry by staining cells with propidium iodine; n = 5. Statistical analysis was performed using the unpaired t-test and two-way ANOVA with Sidak’s multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.001. The bars and whiskers represent the means ±SD of different independent experiments.