Figure 7.

Inflammatory responses are more pronounced in infected osteocytes than in osteoblasts. (A) Infected cells were incubated for 24 h and IL-6 was measured by ELISA in cell free supernatants. Uninfected values were subtracted from infected values (pg/10⁶cells); n = 3. (B) Infected cells were incubated for 24 h. Next, extracted LMs were analyzed by UPLC-MS–MS (pg/10⁶cells); n = 6. Statistical analysis was performed using unpaired t-test with Welch’s correction: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. The bars and whiskers represent the means ±SEM of different independent experiments. −: control cells and +: infected cells.