FIGURE 2.

Effect of imoxin on protein levels of ER stress-related markers stimulated by palmitate in C2C12 myotubes. The cells were treated with imoxin (IMX) at 5 μM with/without 0.5 mM palmitate (PA) for 24 h. Protein level of each biomarker was measured by western blot. (A) Representative band images and (B–D) quantification data are shown. Values are presented as mean ± SEM (N = 3). Main effects and interactions were analyzed by two-way ANOVA (IMX x PA), followed by Tukey’s post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the BSA-control group; #p < 0.05 and ###p < 0.001 compared with the PA only group.