FIGURE 3.

Effect of palmitate and Imoxin on p-JNK expression in C2C12 myotubes. The cells were treated with imoxin (IMX) at 5 μM with/without 0.5 mM palmitate (PA) for 24 h. Protein level of p-JNK (p-p54/p46) was measured by western blot. Representative band images are shown on the left panel and quantification of each phosphorylation site and their summation is shown on the right panel. Differences between treatments were analyzed by two-way ANOVA (IMX x PA). Values are presented as mean ± SEM (N = 3). *p < 0.05 and **p < 0.01 compared with the BSA-control group; #p < 0.05 and ##p < 0.01 compared with the PA only group.