FIGURE 5.

Effect of imoxin on insulin-stimulated p-IRβ and IRS1 phosphorylation in presence or absence of palmitate in C2C12 myotubes. The cells were treated with imoxin (IMX) at 5 μM with/without 0.5 mM palmitate (PA) for 24 h. Protein level of each biomarker was measured by western blot. (A) Representative band images and (B–E) quantification data of each biomarker are shown. Differences between treatments were analyzed by three-way ANOVA (IMX x PA x insulin). Values are presented as mean ± SEM (N = 3). *p < 0.05 and **p < 0.01 by Tukey’s post hoc analysis after three-way ANOVA.