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. 2022 Jul 29;2022:7671324. doi: 10.1155/2022/7671324

Figure 7.

Figure 7

miR-494-3p aggravates pathological changes, upregulates ACSL4, and downregulates TH in the substantia nigra and corpus striatum of the MPTP-induced PD mouse model. PD mouse model was established through MPTP induction, and then, PD model mice were addressed with miR-494-3p antagomir, miR-494-3p agomir, or ferrostatin-1. (a and b) qRT-PCR was utilized to analyze miR-494-3p (a) and REST (b) levels. (c) H&E staining determined the pathological changes in the substantia nigra and corpus striatum in the MPTP-induced PD mouse model. Magnification: 400×, scale bar = 50 μm. (d) ACSL4 expression was assessed using an immunofluorescence assay. Magnification: 200×, scale bar = 100 μm. (e) TH expression was identified using an immunohistochemistry assay. Magnification: 400×, scale bar = 50 μm. Results were representative data from triplicate experiments, and data are means ± SD. Each group contained eight mice. ∗∗∗P < 0.001 vs. sham group; ##P < 0.01 and ###P < 0.001 vs. MPTP+NC group; &&P < 0.01 vs. MPTP group.