Table 1.
Genome assembly statistics for the diploid, tetraploid and hexaploid Avena species
| A. longiglumis, CN 58138 (2n = 2x = 14), AlAl | A. insularis, CN 108634 (2n = 4x = 28), CCDD | A. sativa ssp. nuda cv. Sanfensan (2n = 6x = 42), AACCDD | |
|---|---|---|---|
| Illumina (Gb) | 204.68 | 451.89 | 649.68 |
| ONT (Gb) | 268.74 | 481.39 | – |
| ONT ultralong (Gb) | – | – | 1260.30 |
| Hi-C (Gb) | – | 816.93 | 1312.83 |
| IsoSeq (Gb) | 25.74 | 49.94 | 81.14 |
| Total assembly size (bp) | 3,736,548,545 | 7,519,018,440 | 10,757,433,345 |
| Longest contigs (bp) | 29,014,927 | 30,586,412 | 313,872,778 |
| Number of contigs | 956 | 2,732 | 436 |
| N50 contig length (bp) | 7,297,603 | 5,637,473 | 75,273,016 |
| L50 contig count | 160 | 399 | 44 |
| N90 contig length (bp) | 2,123,884 | 1,477,395 | 16,452,443 |
| L90 contig count | 523 | 1,374 | 160 |
| Number of contigs per (sub)genome | A: 943 | – | A: 87 |
| – | C: 1,019 | C: 159 | |
| – | D: 848 | D: 77 | |
| Total: 943 | T: 1,867 | T: 323 | |
| Sequences assigned per (sub)genome (bp) | A: 3,708,832,268 | – | A: 3,346,241,509 |
| – | C: 4,020,068,809 | C: 4,094,063,504 | |
| – | D: 3,131,824,267 | D: 3,215,854,646 | |
| T: 3,708,832,268 | T: 7,151,893,076 | T: 10,656,159,659 | |
| Genome completeness (BUSCO) | 98.51 | 99.32 | 99.44 |
| Repeats (%) | 86.83% | 87.11% | 86.95% |
| Protein-coding genes | A: 43,477 | – | A: 41,433 |
| – | C: 43,243 | C: 36,283 | |
| – | D: 43,911 | D: 41,633 | |
| T: 43,477 | T: 89,995 | T: 120,769 | |
| Pseudogenes | A: 14,058 | – | A: 23695 |
| – | C: 20,847 | C: 28056 | |
| – | D: 17,766 | D: 24,009 | |
| T: 14,058 | T: 40,027 | T: 76,646 |
A, A subgenome; C, C subgenome; D, D subgenome; T, total.