Extended Data Fig. 9. Flow cytometry gating strategy and Ki67 measurements.

a-b) CD3⁺ CD4⁺ and CD3⁺ CD8⁺ subpopulation cell numbers + /- ROCK inhibition (Y27632). Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****p = 1.00E^{^−6}, *p = 0.0387, 0.0117, 0.0201, 0.0226. n = individual LNs where Day 0 (n = 28), Day 0^Y27 (n = 28), Day 5 (n = 7), Day 5^Y27(n = 9) over 2 independent experiments. c) Example of Ki67⁺ FRCs cell plots and histogram of Ki67 geometric mean, comparing immunization and Y27 treatments. d) Quantification of Ki67⁺ FRCs. Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons. ****p = 1.00E^{^−6}, ***p = 1.00E^{^−5}. n = individual LNs where Day 0 (n = 28), Day 0^Y27 (n = 29), Day 5 (n = 14), Day 5^Y27(n = 15) over 5 independent experiments. This figure supports Fig. 8.

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