Fig. 8. Mechanical tension gates FRC proliferation in vivo.

a, Schematic of immunization and Y27 treatment timeline. Draining LNs (Dr) and non-draining LNs (nDr). b, LN mass and cellularity. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10⁻⁶. n indicates LNs on day 0 (n ≥ 28), day 0^Y27 (n ≥ 28), day 5 (n ≥ 7), and day 5^Y27(n ≥ 9) over two independent experiments. c, CD45⁺ and CD45⁻ cell numbers. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10⁻⁶. d,e, CD19⁺ and CD3⁺ cell numbers and percentages of live. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10⁻⁶. f, Ratio of the percentage of CD19⁺ and CD3⁺ compared to day 0 steady state. Box plots indicate median, interquartile range, and minimum/maximum. Dotted line marks control. Mann–Whitney test (two tailed), **P = 0.0033. g, Total number of LECs (left), BECs (middle), and FRCs (right) after IFA/OVA immunization, ± ROCK inhibition (Y27632). Box plot indicates median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10⁻⁶, **P = 0.0016, *P < 0.05. h, Scatter plots of FRC numbers against initial recoil velocity. Each dot represents the mean ± s.d. for both Y27 (Figs. 8g and 3f) and ΔPDPN (Figs. 7m and 6h) cell number and initial recoil velocity.

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