Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 28;23(8):1256–1272. doi: 10.1038/s41590-022-01271-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, Automated B cell subset identification by FlowSOM. Minimal spanning trees of a representative HC and a patient with pRD are shown. Metaclusters, corresponding to individual B cell subsets are indicated by the background color of the nodes, numbers (M0–15) and marker expression pattern. b, B cell subset composition. Metaclusters from concatenated live B cells of four HCs and four patients with pRD were projected onto t-SNE space and assigned as in a. c, Frequencies of the specific B cell subsets. Circles represent antigen-experienced donors, HCs (HC-Ag, n = 18) and patients with pRD (pRD-Ag, n = 13); triangles represent antigen-naive infant donors (HC-N, n = 4; pRD-N n = 1). Subsets comprising Trans, CD38^int, CD38⁺, CD38⁻, CD27⁺, DN and ASC populations are depicted with different backgrounds. Statistical analyses were performed on individuals with antigens (HC-Ag versus pRD-Ag) (Mann–Whitney U-test with Holm–Šídák multiple comparison). Asterisks indicate new B cell subsets for a–c. d, Activation of peripheral blood B cells. Surface expression of CD69, CD25, HLA-DR, CD80, CD86, CD21 and CXCR5 in CD38^int, CD38⁻ and CD27⁺ B cells is shown as histograms. Filled gray histograms depict the expression of each marker on the total B cells from an HC. Dark gray and red lines represent the expressions of each marker in the indicated compartments (CD38^int, CD38⁻ or CD27⁺) from an HC and a patient with pRD, respectively. Geometric mean fluorescence intensities (gMFIs) are indicated for each HC (black) and pRD B cell subsets (red) and HC total B cells (gray). e, Expression of activation markers. gMFI of each marker from CD38^int, CD38⁻ and CD27⁺ compartments were normalized by donors to the gMFIs of the total B cells from the HC(s) used in each experiment. Data are shown as mean ± s.e.m. with individual values. Therefore, changes in the expression by compartments in HC-Ag (gray, n = 14) and patients with pRD-Ag (red, n = 6–8) are shown as relative to 1 (green dashed line), which represents marker expression level in the total B cells of HCs. Data were analyzed by Mann–Whitney U-test.

Source data