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. 2022 Jul 28;23(8):1256–1272. doi: 10.1038/s41590-022-01271-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a) Hematoxylin and eosin staining of spleen sections from a HC and a pRD-Ag subject. 40x magnifications. (b) Hematoxylin and eosin staining of spleen sections from a HC and a pRD-Ag subject. Follicles are depicted with yellow dashed line. 10x magnifications. (c-f) Chromogenic staining for CD20 (c), CD21 (d), CD4 (e) and Bcl-2(f) in a pRD-Ag subject. 50x magnifications. (g) Cellular distribution shown by DAPI (nucleus) staining by confocal microscopy. 10x magnification. (h-i) Representative multi-color immunofluorescence images for T-bet expression in follicular (Fo) (h) and extrafollicular (EF) (i) areas. Overlay shows CD19 (red), CD4 (green), T-bet (yellow) and DAPI (blue); and CD19 (red), CD4 (green) and T-bet (yellow) expressions are shown separately. 60x magnification. All images were obtained from the spleen sections of a single experiments performed simultaneously for a HC-Ag and a pRD-Ag (P5) subject.

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