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SDS-PAGE analysis (15% polyacrylamide gel) of proteins extracted from wild-type and mutant spores. (A) Lanes: 1, cotEΔ::cat gerE36; 2, gerE36; 3, cotEΔ::cat; 4, wild type (WT); 5, empty lane; 6, gerE36 tasAΩpAGS08. (B) Lysate of cotEΔ::cat sporangia (sprng) 5 h after initiation of sporulation. The arrowhead indicates the 40-kDa species. The arrows indicate the position of TasA. Molecular masses are indicated in kilodaltons.
