FIG. 7.

Western blot analysis (SDS–22% polyacrylamide gel) of TasA in the lysate of sporangia, vegetatively growing cells, and extracts of spores of cotEΔ::cat gerE36 sipWΔ::neo strains. (A) Lane 1 contains an extract of cotEΔ::cat gerE36 spores (to indicate the position of TasA). Lanes 2 through 6 contain preparations of vegetative cells (lane 2), vegetative-cell supernatant (lane 3), sporangia (lane 4), sporangial supernatant (lane 5), or spores (lane 6) from AGS215, in which the direction of transcription of the neomycin resistance gene is aligned with that of sipW. (B) Lanes 1 and 2 contain extracts of spores (lane 1) or sporangia (lane 2) of AGS186, bearing the neomycin resistance gene with the opposite orientation relative to the direction of transcription of sipW. Lane 3 contains an extract of cotEΔ::cat gerE36 spores (to indicate the position of TasA). The arrows at the side of the blot indicate the positions of TasA and pre-TasA. The arrowhead indicates the position of the putative breakdown product of TasA. sprng, sporangia; sup, supernatant; veg, vegetative. Molecular masses are indicated in kilodaltons.