TABLE 1.
Strains and plasmids used in this study
| Strains or plasmids | Genotype or description | Source or reference |
|---|---|---|
| B. subtilis | ||
| PY79 | Wild type | 34 |
| AD17 | gerE36 | 5 |
| AD28 | cotEΔ::cat | 8 |
| AD142 | cotEΔ::cat gerE36 | This study |
| RL102 | spo0HΔHindIII-EcoRI::cat trpC2 (168 background) | Losick laboratory collection |
| RL891 | spo0AΔ::erm | 21 |
| SC1159 | spoIIAC1 | 3 |
| PM806 | spoIIGAΔ17 | 21 |
| SC500 | spoIIIGΔ1 | 4 |
| SAB50 | spoIVFΔAB::cat | Losick laboratory collection |
| AGS115 | tasAΩpAGS08 | This study |
| AGS127 | gerE36 tasAΩpAGS08 | This study |
| AGS157 |
sipWΔ::
|
This study |
| AGS175 |
yqxM::
|
This study |
| AGS185 |
cotEΔ::cat gerE36 yqxM::
|
This study |
| AGS186 |
cotEΔ::cat gerE36 sipWΔ::
|
This study |
| AGS200 | spoIIBΔ::erm tasAΩpAGS08 | This study |
| AGS202 | spoVG::Tn917ΩHU265 tasAΩpAGS08 | This study |
| AGS207 | tasAΔ::spc | This study |
| AGS210 | cotEΔ::cat gerE36 tasAΔ::spc | This study |
| AGS215 |
cotEΔ::cat gerE36 sipWΔ::
|
This study |
| AGS219 | yqxM::neo tasAΩpAGS08 | This study |
| E. coli | ||
| DH5α | Cloning host | Laboratory collection |
| BL21(DE3) | Overproduction host | Laboratory collection |
| Plasmids | ||
| pBEST501 | Harbors the neomycin resistance gene | 14 |
| pJL74 | Harbors the spectinomycin resistance gene | 18 |
| pSL1180 | Harbors extensive multiple cloning site | Pharmacia |
| pET24b | Overexpression vector | Novagen |