TABLE 2.

Primers used in this study

Primer	Sequencea	Restriction enzymea
OL58	5′ AAAAAAAACATATGAAGGACAGCACCATGTCTAC 3′	NdeI
OL59	5′ AAAAACTCGAGAAAAAGAGGAGTAGTGCCTG 3′	XhoI
OL72	5′ AAAAAAACTCGAGAGCGGTGTTTCTTCTGCGTG 3′	XhoI
OL74	5′ AAAAAAACTCGAGTTGTGCTGACACTTGTCGTG 3′	XhoI
OL75	5′ AAAAAAATCGATCCGAATACGATGGTCAATTAGATAG 3′	ClaI
OL76	5′ AAAAAAACTCGAGAACGGCAGTACATTTGAGTGG 3′	XhoI
OL77	5′ AAAAAAATCGATCCTCCAACTAAAGCTAATCCTAGTG 3′	ClaI
OL91	5′ AAAAAAAAACTCGAGCTGATCAGCTTCATTGCT 3′	XhoI
OL93	5′ AAAAAAACTCGAGTTTATCCTCGCTATGCGC 3′	XhoI
OL94	5′ AAAAAAAAACATATGGCATTTAACGACATTAAA 3′	NdeI
OL99	5′ AAAAACTCGAGCATATGTTTCGATTGTTTCAC 3′	NdeI

^aThe restriction endonuclease site in each oligonucleotide is underlined, and the enzyme that cuts it is listed at the right.