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. 2022 Aug 5;12:13484. doi: 10.1038/s41598-022-16754-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activated human hepatic stellate cells are divided in two functionally specific subpopulations. (A) UMAP projection of the three hepatic stellate cell clusters. (B) Violin plots of normalised and batch-corrected expression of hepatic stellate cell marker genes. (C) Violin plots of the activity of hepatic stellate cell modules. (D) M-HSC-2, of which the 30 most central genes are seen in the network diagram. In the network diagram, node sizes represent gene centrality in the module (‘kIM’), and edge widths represent the strength of gene–gene co-expression. For the bar plot, gene sets from the Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Reactome databases were queried. Only significant results were plotted (P < 0.05).