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. 2022 Aug 5;5:787. doi: 10.1038/s42003-022-03735-9

Fig. 5. JNJ-1289 inhibits hSMOX in a time-dependent manner.

Fig. 5

a Structure of JNJ-1289. b Inhibition by JNJ-1289 at 2.5 nM hSMOX, 60 pM hPAOX or 10 nM LSD1 in the presence of 30 µM Spm, 20 µM N-AcSpm, or 8 µM H3K4me2 peptide, respectively. JNJ-1289 inhibits hSMOX under these assay conditions with an IC50 value of 50 nM. c Thermal shift analysis of hSMOX reveals that incubation with JNJ-1289 (red line) stabilizes the protein with ΔTm = 11.3 °C compared to DMSO control (blue line). d Inhibition of hSMOX measured using a 0 h or 2 h preincubation of enzyme and JNJ-1289 at 2.5 nM enzyme and 30 µM Spm. The observed IC50 values of 127 and 8 nM at 0 and 2 h enzyme-inhibitor preincubation times, respectively, suggested that JNJ-1289 inhibits hSMOX in a time-dependent manner.