Fig. 2. Gemcitabine-resistant cells show multi-drug resistance and are more invasive than their counterpart-sensitive cells.

a Cell proliferation rate was measured for GR and GS cells treated with different doses of gemcitabine and paclitaxel for 3 days by MTT assays, the data were represented as mean ± SD in four replicates experiments. b 3 × 10⁴ cells/well of GS and GR cells were plated in the Matrigel invasion inserts (24-well size) for 24 h. Invaded cells were counted under the light microscope and the data were represented as mean ± SD performed in triplicate. c Western blot analysis of GR/shCD44 clones for the expression of CD44 and EMT makers. d GR and GR/shCD44 clones were treated with different doses of gemcitabine for 3 days, the cell proliferation rate was compared by MTT assays, and the data were represented as mean ± SD in four replicates experiment. e Matrigel invasion assay compared GR to GR/shCD44 cells, the data were represented as mean ± SD performed in triplicate.