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. 2022 Aug 5;13:4562. doi: 10.1038/s41467-022-32335-8
MCF7 clone Indel % KO score Indel Genotype
AFDN sg2 clone-4 (M2C4) 95 95 −7, +1 Added C or G causes a heterozygous indel resulting in a 39 aa or 62 aa truncated protein
AFDN sg1 clone-1 (M1C1) 99 99 −1 Loss of A results in a 41 aa truncated protein
AFDN sg1 clone-3 (M1C3) - - - -
AFDN sg1 clone-6 (M1C6) 91 85 +1 Added G results in a 49 aa truncated protein
SCRIB sg1 (S1C6) 94 77 −1, +1, −2 Loss of T results in a 134 aa truncated protein; Added G in a 121 aa truncation; Loss of CT a 120aa protein
SCRIB sg1 (S1C17) 97 97 +1 Added T results in a 121 aa truncated protein
SCRIB sg2 (S2C13) 96 96 −1 Loss of T results in a 134 aa truncated protein
SCRIB sg2 (S2C16) 98 98 −2 Loss of TT results in a 120 aa truncated protein