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. 2022 Aug 5;13:4567. doi: 10.1038/s41467-022-32349-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Hydrodynamic particle size of PCoA@M over time in different media. Data are presented as the means ± s.d. (n = 3 independent samples). b Haemolysis rate of erythrocytes after incubation with different concentrations of probe. Inset: bright field image and micrograph of the cell smear made with the precipitate after incubation. c CCK-8 cytotoxicity of 4T1 cells incubated with different concentrations of PCo@M. Data are presented as the means ± s.d. (n = 6 independent samples). d Survival rate of 4T1 cells incubated and treated with different probes. Data are presented as the means ± s.d. (n = 6 independent samples). Statistical differences were calculated using two-tailed Student’s t test, *: p < 0.05; **: p < 0.01; ***: p < 0.001. e, f Western blot analysis of HSP after treating 4T1 cells with different probes and at different pH values. g Immunofluorescence imaging for the detection of α4 and β1 antigens on the macrophage membrane. Images are representative of three biologically independent samples. Scale bar: 50 μm. h Confocal imaging of Dil-labelled PCoA@M and PCoA@D incubated with RAW 264.7 cells for 5 h. Scale bar: 50 μm. i Colocalized images of Dil-labelled PCoA@M and 4T1 cells incubated for 2, 3, 5, 7, 9, 11, and 13 h. Scale bar: 50 μm.