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. 2022 Aug 5;13:4567. doi: 10.1038/s41467-022-32349-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Treatment process. b, c Temperature change curves over time and thermal images of tumours after injecting of in situ 4T1 tumour-bearing mice with PBS, P@M, PCo@M, and PCoA@M and treated with NIR. Colour scale: black to red represents the temperature of the tumour from low to high. Data are presented as the means ± s.d. (n = 3 mice per group). d Fluorescence imaging of 4T1 tumour sections after different Dil-labelled probes were injected into mice. Scale bar: 400 μm. e–i TUNEL, HE, Bax, Bcl-2, and HSP90 immunofluorescence staining at the tumour site. Red arrow in f: vacuolization in the cell; blue arrow: cell necrosis; yellow arrow: cell atrophy; purple arrow: separation of the nucleus and cytoplasm. Images are representative of three biologically independent mice. Scale bars in e, g, h and i: 100 μm; scale bar of HE images in f: 100 μm; scale bar of tumour images in f: 5 mm.