Fig. 8. Long-term therapy of in situ tumours.

a WSP-1 immunofluorescence staining images of the tumour site after different treatments. Images are representative of three biologically independent mice. Scale bar: 200 μm. b Relative tumour volume changes. Data are presented as the means ± s.d. (n = 5 mice per group). Statistical differences were calculated using two-tailed Student’s t test, *: p < 0.05; **: p < 0.01; ***: p < 0.001. c–f Mouse weight changes, bright field images of the mice and tumours and tumour quality analysis. Data are presented as the means ± s.d. (n = 5 mice per group). Statistical differences were calculated using two-tailed Student’s t test, *: p < 0.05; **: p < 0.01; ***: p < 0.001. g, h Bouin’s trichrome fixed lung tissue of mice after sacrifice and the corresponding HE staining images. Scale bar: 4 mm. i, j Ki-67 immunofluorescence staining images of the tumour site at different times after different treatments. Images are representative of three biologically independent mice. Scale bar: 100 μm.