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. 2022 Jul 25;54(7):1024–1037. doi: 10.1038/s12276-022-00806-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Compared with circANRIL, circROCK1 was abundantly enriched in AGO2 by using IgG as an internal control. ^n.sp > 0.05 and ^***p < 0.001 c A Venn diagram exhibiting overlapping miRNAs that might interact with circROCK1-E3/E4 and presented in GSE65071. d Expression of miR-532-5p in osteosarcoma tissues was illustrated by an in situ hybridization assay. e Expression of miR-532-5p in osteosarcoma tissues and paired healthy tissues in the osteosarcoma-related genome-wide study GSE65071. Probe ID 49 represents miR-532-5p. f Spearman correlation analysis showed that circROCK1-E3/E4 was inversely correlated with miR-532-5p. r = –0.5406 and p < 0.0001. g Expression levels of miR-532-5p in osteosarcoma cell lines were confirmed by an RT–qPCR assay. h The subcellular localization of circROCK1-E3/E4 and miR-532-5p was confirmed by a FISH assay. i The targeted binding effect and binding sites between circROCK1-E3/E4 and miR-532-5p were tested by a luciferase assay. ^n.sp > 0.05 and ^*p < 0.05. j, k Expression of miR-532-5p after up- and downregulation of circROCK1-E3/E4 was analyzed by RT–qPCR. ^n.sp > 0.05 and ^**p < 0.01. l, m Relative expression of circROCK1-E3/E4 after different miR-532-5p interventions was measured by RT–qPCR. ^n.sp > 0.05. All data are presented as the mean ± SD from three independent experiments.