Table 1.
Primer and oligonucleotide sequences used in this study.
| Gene | Primer (sense) | Primer (antisense) |
|---|---|---|
| hsa_circ_0047061 | CTCTTCTTCAAAAATGACCAGTGGG | TTCTTCTACACCATTTCGCCCT |
| hsa_circ_0047060 | AAATGGAGCAGAAGTGCAGAACC | CAAACTTTCCTGCTCTTCATCCAA |
| hsa_circ_0108012 | GGAGCAGAAGTGCAGATACT | GCAAACTTTCCTGCAAGCTTTTAT |
| hsa_circ_0107991 | GCTGCAACTGGAACTCAACC | TTAACAGCCGCTTGCATGTC |
| hsa_circ_0108010 | GAGCAGAAGTGCAGAACCTCA | CTGCATTGGAGCTAGTTCTGT |
| hsa_circ_0108011 | AAATCACGACATGGCCCCTC | CGTGGTGGCTCACACTTCTT |
| hsa_circ_0108016 | AGCTTGCTGTAGCACCAGTT | AGGGAATGTTTCTTCCTCTCCTT |
| circROCK1-E3/E4 | TGGGTTGTTCAGATAAAGACACA | TGCACCTCTACCAATCACCT |
| hsa_circ_0107997 | AATGAAGGACAGATGCGGGAG | CTGCTCCAGTTGCAGGGTTAG |
| hsa_circ_0108001 | GTGGCAATGTGTGCTCGAAT | AGCATGTCTTGAGCCTCTTTTC |
| hsa_circ_0047062 | GCCTGAAAAATGGGCACGAT | ATGCCTTACCTGTGAATAAAACC |
| hsa_circ_0047056 | GTTTTTAAGCCACCCCCTGC | CACATCTCCTTGGGTTACAGGT |
| hsa_circ_0107992 | CCTTAAAACACAGAGAAACTCTTGC | TGTTCTTCCAGAAGGCCTCG |
| cANRIL | GCTGGGATTACAGGTGTGAGACACC | GAATCAGAATGAGGCTTATTCTTCTCATC |
| ROCK1 mRNA | AAGAGGGCATTGTCACAGCA | AGCATCCAATCCATCCAGCA |
| QKI mRNA | ACCTGCAGCAGAAGGAGAAG | AAGGCAAGGGCTGGTGATTT |
| PTEN mRNA | TCCCAGACATGACAGCCATC | TGCTTTGAATCCAAAAACCTTACT |
| GAPDH mRNA | AACGGATTTGGTCGTATTGGG | CCTGGAAGATGGTGATGGGAT |
| β-Actin mRNA | GATTCCTATGTGGGCGACGA | TGTAGAAGGTGTGGTGCCAG |
| ROCK1 intron 2 | TAGTGGGCGCAGTTAGACAC | CCCTGTGGAAGAACCTTGCT |
| ROCK1 intron 4 | TGAAGCTGTGGCCATGTTACT | CAAAACCCCACTTGACCCCA |
| miR-532-5p RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACGGTC | |
| miR-532-5p | GCGGCGGCATGCCTTGAGTGTAG | ATCCAGTGCAGGGTCCGAGG |
| U6 RT | CGAGCACAGAATCGCTTCACGAATTTGCGTGTCAT | |
| U6 | CGAGCACAGAATCGCTTCA | CTCGCTTCGGCAGCACATAT |
| siRNA | Sense | Antisense |
| siNC | UUCUCCGAACGUGUCACGU dTdT | ACGUGACACGUUCGGAGAA dTdT |
| sicircROCK1-1 | GTTGTTCAGATAAAGACACAAUU | TTGTGTCTTTATCTGAACAACUU |
| sicircROCK1-2 | GGTTGTTCAGATAAAGACACAUU | TGTGTCTTTATCTGAACAACCUU |
| siQKI-1 | GCAUCUAAAUGAAGAUUUAUU | TAAATCTTCATTTAGATGCUU |
| siQKI-2 | GGUACUUUAUGUAUAAUUAUU | TAATTATACATAAAGTACCUU |
| siPTEN-1 | GAUGAUACAUGUAAAUUAAUU | TTAATTTACATGTATCATCUU |
| siPTEN-2 | GAAUGGAUUUGAUACUUUAUU | TAAAGTATCAAATCCATTCUU |
| miR-532-5p mimic | CAUGCCUUGAGUGUAGGACCGU | GGUCCUACACUCAAGGCAUGUU |
| miR-532-5p inhibitors | ACGGUCCUACACUCAAGGCAUG | |
| miR NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
| Inh NC | CAGUACUUUUGUGUAGUACAA | |