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. 2022 Aug 5;12:13508. doi: 10.1038/s41598-022-17752-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gold nanoparticle uptake in two-dimensional and three-dimensional models (a,b) Darkfield images of (a) a control and (b) a docetaxel-treated monolayer of PC-3 cells. (c,d) 10 µm spheroid sections of (c) control and (d) docetaxel-treated PC-3 cells treated with docetaxel and gold nanoparticles. Inset is hyper spectral spectrum of gold nanoparticles and cells. Scale bar is 40 µm. (e,f) Uptake of gold nanoparticles in (e) monolayer and (f) spheroids of PC-3 and LNCaP with and without docetaxel. Error bars are the 95% confidence interval from three independent experiments. ns indicates no significance, * indicates 0.01 < P < 0.05, ** indicates 0.001 < P < 0.01.